Description
Glycine Oxidase H244K, Bacillus subtilis recombinant is available at Gentaur for Next week delivery.
≥90% Pure Active Bacillus subtilis recombinant Glycine Oxidase H244K which shows a higher substrate specificity ratio for glycine versus sarcosine
Biomolecule/Target: Glycine Oxidase
Alternates names: Glycine oxidase, glycine oxygen oxidoreductase (deaminating), GO
Synonyms: Glycine oxidase, glycine oxygen oxidoreductase (deaminating), GO
Background Information: Glycine oxidase (GO) from Bacillus subtilis (EC 1.4.3.19) is a homotetrameric flavin-dependent oxidoreductase. Each GO monomer is non-covalently bound to flavin adenine dinucleotide. GO catalyzes oxidative deamination of various primary and secondary amines (e.g. glycine, sarcosine, N-ethylglycine) and some D-amino acids (e.g. D -alanine, D -proline, D -valine) to the corresponding α-keto acids and hydrogen peroxide. Primarily, glycine oxidase catalyzes the oxidation of glycine in the biosynthesis of thiamine. The variant H244K shows a higher substrate specificity ratio for glycine versus sarcosine and a 5-fold improved specific activity in comparison to the wild-type.
Reconstitution Instructions: N/A
NCBI Gene Symbol: thiO
Gene ID: 939377
NCBI Accession: O31616
Additional Information
Size: |
250 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Bacillus subtilis |
Recombinant: |
Yes |
Source: |
E. coli |
Purity by SDS-PAGE: |
≥90% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
1200 mU/mg |
Biological activity: |
1200 mU/mg |
Results: |
1200 mU/mg |
Molecular Weight: |
43.1 kDa (1-369 aa, NT His Tag) |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
4.0 mg/ml |
Appearance: |
Liquid |
Handling: |
Centrifuge the vial prior to opening. |