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The HybridoMax Hybridoma Growth Supplement is ready to use. Prepare the serum free Hybridoma growth medium by dissolving 1 bottle of HybridoMax (11 ml) into 1 Liter of D -MEM F-12 (Dulbecco’s Modified Eagle’s Medium F- 12).
- There is no need to test the medium before use. All compounds and the complete medium are tested in advance by our company.
- The solution is sterile-filtered.
- The cell compartment will comprise about 20 ml cell suspension with the individual secreted product.
- Due to osmotic flux of liquid from the medium compartment to the cell compartment, the total volume might be slightly increased.
- The cell compartment harvest can be further purified according to the recommended purification method for your secreted product.