Teriffic Broth Gentaur
Terrific Broth is used with glycerol in cultivating recombinant strains of E. coli. Terrific broth is a highly enriched medium for improving yield in plasmid bearing E. coli. Recombinant strains have an extended growth phase in this medium. The addition of tryptone and yeast extract in the medium will allow higher plasmid yield per volume. Glycerol is used as a carbohydrate source in this formulation. Unlike glucose, glycerol is not fermented to acetic acid.
- The formula per 50.8 g which is used to prepare 1 L of growth medium contains:
- Casein Digest Peptone 12.0 g
- Yeast Extract 24.0 g
- Potassium Phosphate, Monobasic 2.3 g
- Potassium Phosphate, Dibasic 12.5 g