Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) is a house keeping gene
and served as internal control for gene and protein expression. GAPDH is a key enzyme in the
biological process of glycolysis which breakdowns a monosaccharide (generally glucose) into
simpler components, including pyruvate.
Applications and Suggested Dilutions (Sensitivity):
- • Western blot: 1:1,000-1:2,000
- • ELISA: 1:1,000,000
- • Immunoaffinity purification of GAPDH
- • Immunohistochemistry: 1:100-1:200
- • Immunoprecipitation: 1:1,000
- • Optimal working dilutions must be determined by end user
Source: Mouse monoclonal antibody produced by hybridoma clones.
Format/Reconstitution: Liquid immunoglobulin was purified from ascites by protein G column
and stored in PBS with 0.2% BSA and 0.09% sodium azide. No reconstitution is necessary.
Immunogen/Sequence: Glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle.
Mol. Wt. Of Antigen: 36-38 kDa
Protein Specificity: Western blot shows single band of Glyceraldehyde-3-phosphate
dehydrogenase (GAPDH) in major organs of human, mouse, rat and rabbit.
Tissue/cell line Specificity and Cellular Localization: All tissues in Cytoplasmic.
Species Specificity: Human, rat, mouse (weak), rabbit, monkey, chicken, frog, and fish skeletal
Storage/handling: Maintain at 2-8˚C in undiluted aliquots for at least 6 months after da
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