BioVision Proteins
B. subtilis Recombinant, Oxalate oxidase (OxOx)
- SKU:
- P1091-20
Description
B. subtilis Recombinant, Oxalate oxidase (OxOx) is available at Gentaur for Next week delivery.
Converts oxalate to formate and CO2 in an O(2)-dependent reaction
Biomolecule/Target: oxdC
Alternates names: OxO, OxOx, OxO_r
Synonyms: OxO, OxOx, OxO_r
Background Information: Oxalate oxidase is a member of the cupin superfamily. It catalyzes the breakdown of oxalate to hydrogen peroxide and carbon dioxide. Oxalate oxidase and oxalate decarboxylase are two similar enzymes that share similar structural and sequence identity. Both enzymes use oxalate as substrate and manganese ions as cofactors. BioVision’s oxalate oxidase is derived from B. subtilis oxalate decarboxylase by introducing three mutations: S161D, E162A and N163S in the active-site loop.
Reconstitution Instructions: Reconstitute to 2 mg/mL in sterile water
NCBI Gene Symbol: oxdC
Gene ID: 938620
NCBI Accession: O34714
Additional Information
Size: |
20 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Bacillus subtilis |
Recombinant: |
Yes |
Source: |
E. Coli |
Purity by SDS-PAGE: |
≥90% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
Measured by the conversion of 1 µmole of oxalate to CO2 and H2O2 per minute at pH 4.5 and 25C. |
Biological activity: |
≥ 230 mU/mg and is defined as the amount of enzyme that catayze the conversion of converts 1 µmole of oxalate to CO2 and H2O2 per minute at pH 4.5 and 25C. |
Results: |
≥ 230 mU/mg |
Molecular Weight: |
43.6 kDa |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Freeze dried |
Handling: |
Centrifuge the vial prior to opening. |