Glycine Oxidase H244K, Bacillus subtilis recombinant

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SKU:
7845-250
€185
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Description

Glycine Oxidase H244K, Bacillus subtilis recombinant is available at Gentaur for Next week delivery.

≥90% Pure Active Bacillus subtilis recombinant Glycine Oxidase H244K which shows a higher substrate specificity ratio for glycine versus sarcosine

Biomolecule/Target: Glycine Oxidase

Alternates names: Glycine oxidase, glycine oxygen oxidoreductase (deaminating), GO

Synonyms: Glycine oxidase, glycine oxygen oxidoreductase (deaminating), GO

Background Information: Glycine oxidase (GO) from Bacillus subtilis (EC 1.4.3.19) is a homotetrameric flavin-dependent oxidoreductase. Each GO monomer is non-covalently bound to flavin adenine dinucleotide. GO catalyzes oxidative deamination of various primary and secondary amines (e.g. glycine, sarcosine, N-ethylglycine) and some D-amino acids (e.g. D -alanine, D -proline, D -valine) to the corresponding α-keto acids and hydrogen peroxide. Primarily, glycine oxidase catalyzes the oxidation of glycine in the biosynthesis of thiamine. The variant H244K shows a higher substrate specificity ratio for glycine versus sarcosine and a 5-fold improved specific activity in comparison to the wild-type.

Reconstitution Instructions: N/A

NCBI Gene Symbol: thiO

Gene ID: 939377

NCBI Accession: O31616

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Additional Information

Size:
250 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Bacillus subtilis
Recombinant:
Yes
Source:
E. coli
Purity by SDS-PAGE:
≥90%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
N/A
Endotoxin Level:
N/A
Activity (Specifications/test method):
1200 mU/mg
Biological activity:
1200 mU/mg
Results:
1200 mU/mg
Molecular Weight:
43.1 kDa (1-369 aa, NT His Tag)
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
4.0 mg/ml
Appearance:
Liquid
Handling:
Centrifuge the vial prior to opening.
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