BioVision Proteins
D-amino acid oxidase, human recombinant
- SKU:
- P1052-50
Description
D-amino acid oxidase, human recombinant is available at Gentaur for Next week delivery.
Enzyme that regulates the level of the neuromodulator D-serine in the brain.
Biomolecule/Target: DAO
Alternates names: DAMOX, DAO, OXDA
Synonyms: DAMOX, DAO, OXDA
Background Information: D-amino-acid oxidase (DAAO) is a peroxisomal enzyme which uses flavin adenine dinucleotide (FAD) as a cofactor and oxidizes D-amino acids to the corresponding imino acids, producing ammonia and hydrogen peroxide. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. It has been suggested that it is involved in acid base balance in the kidney or it could act as a detoxifying agent which removes D-amino acids accumulated during aging. Recombinant human DAAO protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Reconstitution Instructions: N/A
NCBI Gene Symbol: DAO
Gene ID: 1610
NCBI Accession: P14920
Additional Information
Size: |
50 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
E. coli |
Purity by SDS-PAGE: |
> 90% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
Measured by its ability to oxidatively deaminate 1.0 umole of D-alanine to pyruvate per minute at pH 8.5 at 37C, in the presence of catalase. |
Biological activity: |
Specific activity is > 3.5 units/mg, in which one unit will oxidatively deaminate 1.0 umole of D-alanine to pyruvate per minute at pH 8.5 at 37C, in the presence of catalase. |
Results: |
> 3.5 units/mg |
Molecular Weight: |
41.6 kDa |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Liquid |
Handling: |
Centrifuge the vial prior to opening. |
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