BioVision Products
Human CellExp™ PD-L1 /CD274 /B7-H1, mouse recombinant
- SKU:
- 7428-50
Description
Human CellExp™ PD-L1 /CD274 /B7-H1, mouse recombinant is available at Gentaur for Next week delivery.
Biomolecule/Target: PD-L1 /CD274 /B7-H1
Alternates names: PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
Synonyms: PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
Background Information: Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
NCBI Gene Symbol: CD274
Gene ID: 60533
NCBI Accession: Q9EP73
Additional Information
Size: |
50 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Mouse |
Recombinant: |
Yes |
Source: |
HEK293 cells |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
<1 EU/μg by LAL method |
Activity (Specifications/test method): |
N/A |
Biological activity: |
Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse PDL1 at 1 μg/ml (100 μl/well) can bind biotinylated Mouse PDCD1 / PD1 with a linear range of 16 - 500 ng/ml. |
Results: |
Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse PDL1 at 1 μg/ml (100 μl/well) can bind biotinylated Mouse PDCD1 / PD1 with a linear range of 16 - 500 ng/ml. |
Molecular Weight: |
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 25.6 kDa. The predicted N-terminus is Phe 19. DTT-reduced Protein migrates as 40-50 kDa due to glycosylation. |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized |
Handling: |
Centrifuge the vial prior to opening. |