sFas Ligand, human recombinant

(No reviews yet) Write a Review
SKU:
7140-50
$719.07
Frequently bought together:

Description

sFas Ligand, human recombinant is available at Gentaur for Next week delivery.

Leads to down-regulation of the immune response

Biomolecule/Target: sFas Ligand

Alternates names: soluble Fas Ligand (sFasL), TNFSF6, CD95L, Apo I Ligand, APTL

Synonyms: soluble Fas Ligand (sFasL), TNFSF6, CD95L, Apo I Ligand, APTL

Background Information: Fas Ligand (FasL) is a member of the TNF superfamily that is expressed on the cell surface of activated T cells. Binding of FasL to Fas Receptor triggers apoptosis in Fas-bearing cells. FasL has the ability to kill T cells and activated B cells which leads to down-regulation of the immune response. The mechanism of Fas induced apoptosis involves recruitment of procaspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. Both human and murine sFasL are fully active on human and murine cells. Recombinant human soluble Fas Ligand is a 17.9 kDa protein comprising the TNF homologous region of FasL and contains an 8 residue N-terminal His-Tag.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

NCBI Gene Symbol: FASL

Gene ID: 355

NCBI Accession: P25445

View AllClose

Additional Information

Size:
50 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Human
Recombinant:
Yes
Source:
CHO cells
Purity by SDS-PAGE:
≥95%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
HPLC
Endotoxin Level:
< 0.1 ng/μg of protein (<1EU/μg).
Activity (Specifications/test method):
Determined by its ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED50 for this effect is ≤10.0 ng/ml, corresponding to a specific activity of ≥ 1 x 105 units/mg.
Biological activity:
Determined by its ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED50 for this effect is ≤10.0 ng/ml, corresponding to a specific activity of ≥ 1 x 105 units/mg.
Results:
N/A
Molecular Weight:
17.9 kDa
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
N/A
Appearance:
Lyophilized powder
Handling:
Centrifuge the vial prior to opening.
View AllClose

0 Reviews

View AllClose