sFas Receptor, human recombinant

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SKU:
7141-50
€185
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Description

sFas Receptor, human recombinant is available at Gentaur for Next week delivery.

Leads to down-regulation of the immune response

Biomolecule/Target: sFas Receptor

Alternates names: soluble Fas receptor (sFasR), TNFRSF6, CD95, Apo I, Fas Antigen

Synonyms: soluble Fas receptor (sFasR), TNFRSF6, CD95, Apo I, Fas Antigen

Background Information: Fas and Fas Ligand (FasL) belong to the TNF superfamily and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. sFasR is capable of inhibiting FasL induced apoptosis by acting as a decoy receptor that serves as a sink for FasL. The full length Fas (receptor) is a 319 amino acid type I transmembrane protein, which contains a 157 amino acid extracellular domain, a 17 amino acid transmembrane domain, and 145 amino acid cytoplasmic domain. Recombinant human soluble Fas (sFas Receptor) is a 157 amino acid polypeptide (17.6 kDa) corresponding to the TNFR homologous cysteine rich extracellular domain Fas.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

NCBI Gene Symbol: FASL

Gene ID: 355

NCBI Accession: P25445

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Additional Information

Size:
50 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Human
Recombinant:
Yes
Source:
E. coli.
Purity by SDS-PAGE:
≥98%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
HPLC
Endotoxin Level:
< 0.1 ng/μg of protein (<1EU/μg).
Activity (Specifications/test method):
ED50 was determined by its ability to inhibit the cytotoxicity of Jurkat cells using a concentration range of 10-15 μg/ml in the presence of 2ng/ml of hFasL.
Biological activity:
ED50 was determined by its ability to inhibit the cytotoxicity of Jurkat cells using a concentration range of 10-15 μg/ml in the presence of 2ng/ml of hFasL.
Results:
N/A
Molecular Weight:
17.6 kDa
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
N/A
Appearance:
Lyophilized powder
Handling:
Centrifuge the vial prior to opening.
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