Procathepsin B, Human Recombinant

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SKU:
7579-50
£135.37
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Description

Procathepsin B, Human Recombinant is available at Gentaur for Next week delivery.

A lysosomal protease playing a role in protein degradation, arthritis, apoptosis, and cancer.

Biomolecule/Target: Procathepsin B

Alternates names: CTSB, CPSB

Synonyms: CTSB, CPSB

Background Information: Cathepsin B is a lysosomal cysteine protease. It is implicated in playing a role in protein degradation, arthritis, apoptosis, and cancer. Biovision’s Procathepsin B can be auto-catalytically activated to mature Cathepsin B (~28 kDa) by adding an equal volume of 0.2 M NaOAc, 5 mM EDTA pH 4.0 containing fresh 5 mM DTT at room temperature (RT). The enzyme is fully activated within 3-4 h of incubation at RT. After activation mature Cathepsin B is highly auto-proteolytic at pH 4.0, and care must be taken to avoid self-proteolysis. If the activated enzyme is not used immediately, methylmethanthiosulfonate (1 mM final concentration; MeS-SO2Me; MMTS) could be added and sample should be frozen at -80°C. The activity can be then restored by adding L-cysteine (3 M excess over MMTS) to the enzyme solution.

Reconstitution Instructions: Reconstitute in de-ionized water to 1 mg/ml and store at –20°C. Avoid repeated freezing and thawing cycles.

NCBI Gene Symbol: CTSB

Gene ID: 1508

NCBI Accession: P07858

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Additional Information

Size:
50 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Human
Recombinant:
Yes
Source:
E. coli
Purity by SDS-PAGE:
≥95%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
HPLC
Endotoxin Level:
N/A
Activity (Specifications/test method):
> 5000 mU/mg (1 U = 1 µmole/min, after complete activation) of Ac-Arg-Arg-AFC substrate (K140-100) at 37°C in 50 mM Tris, 1 mM EDTA, 10 mM DTT, pH 7.2.
Biological activity:
> 5000 mU/mg (1 U = 1 µmole/min, after complete activation) of Ac-Arg-Arg-AFC substrate (K140-100) at 37°C in 50 mM Tris, 1 mM EDTA, 10 mM DTT, pH 7.2.
Results:
> 5000 mU/mg (1 U = 1 µmole/min, after complete activation)
Molecular Weight:
38.2 kDa (18-339 aa + N-terminal poly-his tag)
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
N/A
Appearance:
Lyophilized
Handling:
Centrifuge the vial prior to opening.
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