Description
Procathepsin B, Human Recombinant is available at Gentaur for Next week delivery.
A lysosomal protease playing a role in protein degradation, arthritis, apoptosis, and cancer.
Biomolecule/Target: Procathepsin B
Alternates names: CTSB, CPSB
Synonyms: CTSB, CPSB
Background Information: Cathepsin B is a lysosomal cysteine protease. It is implicated in playing a role in protein degradation, arthritis, apoptosis, and cancer. Biovision’s Procathepsin B can be auto-catalytically activated to mature Cathepsin B (~28 kDa) by adding an equal volume of 0.2 M NaOAc, 5 mM EDTA pH 4.0 containing fresh 5 mM DTT at room temperature (RT). The enzyme is fully activated within 3-4 h of incubation at RT. After activation mature Cathepsin B is highly auto-proteolytic at pH 4.0, and care must be taken to avoid self-proteolysis. If the activated enzyme is not used immediately, methylmethanthiosulfonate (1 mM final concentration; MeS-SO2Me; MMTS) could be added and sample should be frozen at -80°C. The activity can be then restored by adding L-cysteine (3 M excess over MMTS) to the enzyme solution.
Reconstitution Instructions: Reconstitute in de-ionized water to 1 mg/ml and store at –20°C. Avoid repeated freezing and thawing cycles.
NCBI Gene Symbol: CTSB
Gene ID: 1508
NCBI Accession: P07858
Additional Information
Size: |
50 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
E. coli |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
HPLC |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
> 5000 mU/mg (1 U = 1 µmole/min, after complete activation) of Ac-Arg-Arg-AFC substrate (K140-100) at 37°C in 50 mM Tris, 1 mM EDTA, 10 mM DTT, pH 7.2. |
Biological activity: |
> 5000 mU/mg (1 U = 1 µmole/min, after complete activation) of Ac-Arg-Arg-AFC substrate (K140-100) at 37°C in 50 mM Tris, 1 mM EDTA, 10 mM DTT, pH 7.2. |
Results: |
> 5000 mU/mg (1 U = 1 µmole/min, after complete activation) |
Molecular Weight: |
38.2 kDa (18-339 aa + N-terminal poly-his tag) |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized |
Handling: |
Centrifuge the vial prior to opening. |