Description
PKCmu, Active is available at Gentaur for Next week delivery.
Involved in regulation of glycogen, sugar, and lipid metabolism
Biomolecule/Target:
Alternates names: PKC, Protein kinase C mu
Synonyms: PKC, Protein kinase C mu
Background Information: Protein kinase Cmu (PKCmu) is a novel member of the protein kinase C (PKC) family that differs from the other isoenzymes in structural and enzymatic properties. It is characterized by the presence of a pleckstrin homology (PH) domain and an amino-terminal hydrophobic region and has substrate specificity distinct from other PKC isoforms. PKCmu is a ubiquitous PKC isotype with the highest expression in the thymus, lung and peripheral blood mononuclear cells (1). PKCmu forms a complex in vivo with a phosphatidylinositol 4-kinase and a phosphatidylinositol-4-phosphate 5-kinase. A region of PKCmu between the amino-terminal transmembrane domain and the pleckstrin homology domain is shown to be involved in the association with the lipid kinases (2). PKCmu was also shown to associate with the B cell receptor (BCR) complex and its activity is up-regulated after cross-linking the BCR and CD19 on B cells (3). PKC mu co-precipitates with Syk and phospholipase C-γ 1/2 (PLC γ 1/2) and in vitro phosphorylation of fusion proteins showed that both Syk and PLC γ 1 are potential substrates of PKC mu in vivo. In addition, specific interaction of PKCmu and 14-3-3tau can be shown in the T cell line Jurkat by immunocoprecipitiation and by pulldown assays (4). 14-3-3tau is not a substrate of PKCmu and strongly down-regulates PKCmu kinase activity in vitro. In response to various stimuli, PKC mu activates the mitogen-activated protein kinase (p42/ERK1 MAPK cascade) but does not affect the related c-jun N-terminal kinase or p38 MAPK (5).
Reconstitution Instructions: N/A
NCBI Gene Symbol: PRKD1
Gene ID: 5587
NCBI Accession: Q15139
Additional Information
Size: |
5 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Recombinant: |
Yes |
Source: |
Baculovirus (Sf9 insect cells) |
Purity by SDS-PAGE: |
≥90% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
HPLC |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
680 nmol phosphate incorporated into CREBTIDE substrate peptide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay). |
Biological activity: |
N/A |
Results: |
N/A |
Molecular Weight: |
131.0 kDa |
Storage Temperature: |
-80°C |
Shelf Life: |
12 months |
Concentration: |
5µg/50 µl |
Appearance: |
Liquid |
Handling: |
Centrifuge the vial prior to opening. |