PKAca, Active

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SKU:
7743-5
€185
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Description

PKAca, Active is available at Gentaur for Next week delivery.

Involved in regulation of glycogen, sugar, and lipid metabolism

Biomolecule/Target:

Alternates names: PKA, cAMP-dependent protein kinase catalytic subunit alpha

Synonyms: PKA, cAMP-dependent protein kinase catalytic subunit alpha

Background Information: Most of the effects of cAMP are mediated through the phosphorylation of target proteins on serine or threonine residues by the cAMP-dependent protein kinase (AMPK). The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. The mammalian catalytic subunit has been shown to consist of three PKA gene products: C-α, C-β, and C-γ. Two PKA isoforms exist, designated types I and II, which differ in their dimeric regulatory subunits, designated RI and RII, respectively. Furthermore, there are at least four different regulatory subunits: RI-α, RI-β, RII-α, and RII-β. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. The catalytic subunit C-α of PKA (PKAca) is a member of the Ser/Thr protein kinase family and is a catalytic subunit C-β of AMPK. Tasken et al. assigned the PKAca gene to 19p13.1 (1). Yasuda et al found that protein kinase A is required for long-term potentiation in neonatal tissue and suggested that developmental changes in synapse morphology may underlie the changes in the kinase activity (2). Skalhegg et al generated a null mutation in the major catalytic subunit of PKAca, and observed early postnatal lethality in the majority of C-α knockout mice. Surprisingly, a small percentage of C-α knockout mice, although runted, survived to adulthood. In these animals, compensatory increases in C-β levels occurred in brain whereas many tissues, including skeletal muscle, heart, and sperm, contained less than 10% of the normal PKA activity (3).

Reconstitution Instructions: N/A

NCBI Gene Symbol: PRKACA

Gene ID: 5566

NCBI Accession: NM_002730.

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Additional Information

Size:
5 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Recombinant:
Yes
Source:
Baculovirus (Sf9 insect cells)
Purity by SDS-PAGE:
≥90%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
HPLC
Endotoxin Level:
N/A
Activity (Specifications/test method):
137 nmol phosphate incorporated into Histone H1 per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
Biological activity:
N/A
Results:
N/A
Molecular Weight:
69.0 kDa
Storage Temperature:
-80°C
Shelf Life:
12 months
Concentration:
5µg/50 µl
Appearance:
Liquid
Handling:
Centrifuge the vial prior to opening.
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