Human CellExp™ UPA, human recombinant

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SKU:
7248-50
£135.37
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Description

Human CellExp™ UPA, human recombinant is available at Gentaur for Next week delivery.

A potent marker of invasion and metastasis in a variety of human cancers

Biomolecule/Target: UPA

Alternates names: Urokinase, PLAU, ATF, UPA, URK, u-PA，BDPLT5，QPD

Synonyms: Urokinase, PLAU, ATF, UPA, URK, u-PA，BDPLT5，QPD

Background Information: Urokinase - type plasminogen activator also known as PLAU and UPA, a serine protease with extremely limited substrate specificity, cleaving the sequence Cys – Pro – Gly - Arg560 - Val561 – Val – Gly – Gly – Cys in plasminogen to form plasmin. uPA is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium. The human PLAU is initially synthesized as 431 amino acid precursor with a N-terminal signal peptide (20 residues). The single chain molecule is processed into a disulfide-linked two-chain molecule of different molecular weights. Two forms of the A chain exist, starting at Ser21 (the long form) and Lys156 (the short form). The long and short A chains are unique to the high and low molecular weight forms, respectively. The long A chain contains an EGF-like domain, responsible for binding of the PLAU receptor. The B chain corresponds to the catalytic domain.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in deionized water. Do not vortex. For extended storage, it is recommended to store at -70°C.

NCBI Gene Symbol: uPA

Gene ID: 5328

NCBI Accession: P00749

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Additional Information

Size:
50 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Human
Recombinant:
Yes
Source:
HEK 293 cells
Purity by SDS-PAGE:
≥92%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
HPLC
Endotoxin Level:
< 0.1 ng/μg of protein (<1EU/μg).
Activity (Specifications/test method):
Measured by its ability to cleave a peptide substrate, N - carbobenzyloxy - Gly - Gly - Arg - 7 - amido - 4 - methylcoumarin (Z - GGR - AMC). The specific activity is > 2000 pmoles/min/μg.
Biological activity:
Immobilized Human PLAU, His Tag at 5 μg/mL (100 μl/well) can bind Biotinylated Human uPAR, His, Avitag with a linear range of 0.3-2 ng/mL
Results:
N/A
Molecular Weight:
This protein is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 45.0 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. Please note that the protein is not activated by pr
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
N/A
Appearance:
Lyophilized powder
Handling:
Centrifuge the vial prior to opening.
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