BioVision Products
Human CellExp™ UPA, human recombinant
- SKU:
- 7248-50
Description
Human CellExp™ UPA, human recombinant is available at Gentaur for Next week delivery.
A potent marker of invasion and metastasis in a variety of human cancers
Biomolecule/Target: UPA
Alternates names: Urokinase, PLAU, ATF, UPA, URK, u-PA，BDPLT5，QPD
Synonyms: Urokinase, PLAU, ATF, UPA, URK, u-PA，BDPLT5，QPD
Background Information: Urokinase - type plasminogen activator also known as PLAU and UPA, a serine protease with extremely limited substrate specificity, cleaving the sequence Cys – Pro – Gly - Arg560 - Val561 – Val – Gly – Gly – Cys in plasminogen to form plasmin. uPA is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium. The human PLAU is initially synthesized as 431 amino acid precursor with a N-terminal signal peptide (20 residues). The single chain molecule is processed into a disulfide-linked two-chain molecule of different molecular weights. Two forms of the A chain exist, starting at Ser21 (the long form) and Lys156 (the short form). The long and short A chains are unique to the high and low molecular weight forms, respectively. The long A chain contains an EGF-like domain, responsible for binding of the PLAU receptor. The B chain corresponds to the catalytic domain.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in deionized water. Do not vortex. For extended storage, it is recommended to store at -70°C.
NCBI Gene Symbol: uPA
Gene ID: 5328
NCBI Accession: P00749
Additional Information
Size: |
50 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
HEK 293 cells |
Purity by SDS-PAGE: |
≥92% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
HPLC |
Endotoxin Level: |
< 0.1 ng/μg of protein (<1EU/μg). |
Activity (Specifications/test method): |
Measured by its ability to cleave a peptide substrate, N - carbobenzyloxy - Gly - Gly - Arg - 7 - amido - 4 - methylcoumarin (Z - GGR - AMC). The specific activity is > 2000 pmoles/min/μg. |
Biological activity: |
Immobilized Human PLAU, His Tag at 5 μg/mL (100 μl/well) can bind Biotinylated Human uPAR, His, Avitag with a linear range of 0.3-2 ng/mL |
Results: |
N/A |
Molecular Weight: |
This protein is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 45.0 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. Please note that the protein is not activated by pr |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized powder |
Handling: |
Centrifuge the vial prior to opening. |