BioVision Products
Human CellExp™ SERPIN F1, human recombinant
- SKU:
- 7418-50
Description
Human CellExp™ SERPIN F1, human recombinant is available at Gentaur for Next week delivery.
Has a variety of prpperties including antiangiogenic, antitumorigenic, and neurotrophic properties
Biomolecule/Target: SERPIN F1
Alternates names: SERPINF1, Serpin F1, PEDF, PIG35, EPC-1
Synonyms: SERPINF1, Serpin F1, PEDF, PIG35, EPC-1
Background Information: Serpin F1 (SERPINF1) is also known as Pigment epithelium-derived factor (PEDF), Cell proliferation-inducing gene 35 protein (PIG35). Serpin F1 belongs to the serpin family. Serpin F1 is expressed in quiescent cells. PEDF has a variety of functions including antiangiogenic, antitumorigenic, and neurotrophic properties. Endothelial cell migration is inhibited by SERPINF1/ PEDF. PEDF / SERPINF1 suppresses retinal neovascularization and endothelial cell proliferation. PEDF is also responsible for apoptosis of endothelial cells either through the p38 MAPK pathway or through the FAS/FASL pathway. PEDF also displays neurotrophic functions.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
NCBI Gene Symbol: SERPINF1
Gene ID: 5176
NCBI Accession: P36955
Additional Information
Size: |
50 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
HEK293 cells |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
<1 EU/μg by LAL method |
Activity (Specifications/test method): |
N/A |
Biological activity: |
Measured by its ability to enhance the adhesion of Saos‑2 human osteosarcoma cells to bovine Collagen I coated plate. The ED50 for this effect is typically 0.5-1.6 µg/ml. |
Results: |
Measured by its ability to enhance the adhesion of Saos‑2 human osteosarcoma cells to bovine Collagen I coated plate. The ED50 for this effect is typically 0.5-1.6 µg/ml. |
Molecular Weight: |
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 45.2 kDa. The predicted N-terminus is Gln 20. DTT-reduced Protein migrates as 45-55 kDa. |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized |
Handling: |
Centrifuge the vial prior to opening. |