Human CellExp™ KLK-7 / Kallikrein-7, human recombinant

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SKU:
7510-10
£135.37
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Description

Human CellExp™ KLK-7 / Kallikrein-7, human recombinant is available at Gentaur for Next week delivery.

≥95% pure, active, human cell expressed, human recombinant KLK-7 / Kallikrein-7 - a protein involved in metastasis in several cancers

Biomolecule/Target: KLK-7 / Kallikrein-7

Alternates names: KLK7, Kallikrein-7, PRSS6, SCCE, hK7, hSCCE

Synonyms: KLK7, Kallikrein-7, PRSS6, SCCE, hK7, hSCCE

Background Information: Kallikrein-7 (KLK7) is also known as kallikrein-related peptidase 7, Stratum corneum chymotryptic enzyme, Serine protease 6, KLK7, and PRSS6, is a secreted protein which belongs to the peptidase S1 family and Kallikrein subfamily. KLK7 is secreted as an inactive zymogen in the stratum granulosum layer of the epidermis, requiring proteolytic cleavage of the short N-terminal pro-region to liberate activated enzyme. This may be performed by KLK5 or matriptase, which are in vitro activators of KLK7. Once active, KLK7 is able to cleave desmocollin and corneodesmosin. KLK7 activity is regulated by a number of endogenous protein inhibitors including LEKTI, SPINK6, elafin and alpha-2-Macroglobulin-like 1. Both Zn2+ and Cu2+ ions are also able to inhibit KLK7. Dysregulation of KLK7 has been linked to several skin disorders, and overexpression of KLK7 may provide a route for metastasis in several cancers.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.

NCBI Gene Symbol: KLK7

Gene ID: 5650

NCBI Accession: P49862

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Additional Information

Size:
10 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Human
Recombinant:
Yes
Source:
HEK293 cells
Purity by SDS-PAGE:
≥95%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
N/A
Endotoxin Level:
<1 EU/μg by LAL method
Activity (Specifications/test method):
N/A
Biological activity:
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp) NH2. The specific activity is >130 pmoles / min / μg.
Results:
>130 pmoles / min / μg
Molecular Weight:
This protein is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 26.1 kDa. The predicted N-terminus is Glu 23. DTT-reduced Protein migrates as 29-33 kDa in SDS-PAGE due to glycosylation.
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
N/A
Appearance:
Lyophilized
Handling:
Centrifuge the vial prior to opening.
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