BioVision Products
Human CellExp™ KLK-8 / Kallikrein-8, human recombinant
- SKU:
- 7511-10
Description
Human CellExp™ KLK-8 / Kallikrein-8, human recombinant is available at Gentaur for Next week delivery.
≥95% pure, active, human cell expressed, human recombinant KLK-8 / Kallikrein-8 - serves as a marker of ovarian cancer.
Biomolecule/Target: KLK-8 / Kallikrein-8
Alternates names: KLK8, Kallikrein-8, Neuropsin, Ovasin, NRPN, PRSS19, TADG14, NP, hK8
Synonyms: KLK8, Kallikrein-8, Neuropsin, Ovasin, NRPN, PRSS19, TADG14, NP, hK8
Background Information: Kallikrein-8 (KLK8) is also known as Neuropsin (NP or NRPN), Ovasin, Serine protease 19 (PRSS19), Tumor-associated differentially expressed gene 14 protein (TADG-14), which belongs to the peptidase S1 family and Kallikrein subfamily. KLK8 contains 1 peptidase S1 domain. KLK8 is pH dependence protein and the optimum pH is 8.5, and the protein is active from pH 7-10. KLK8 is expressed at high levels in serum, ascites fluid and tumor cytosol of advanced stage ovarian cancer patients and may serve as a marker of ovarian cancer. KLK8 cleavage of amide substrates following the basic amino acids Arg or Lys at the P1 position, with a preference for Arg over Lys, and the catalytic activity of KLK8 is inhibited by a range of serine protease inhibitors including antipain, aprotinin, leupeptin, benzamidine and soybean trypsin inhibitor.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
NCBI Gene Symbol: KLK8
Gene ID: 11202
NCBI Accession: O60259
Additional Information
Size: |
10 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
HEK293 cells |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
<1 EU/μg by LAL method |
Activity (Specifications/test method): |
N/A |
Biological activity: |
Measured by its ability to inhibit thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >500 pmoles / min / μg. |
Results: |
>500 pmoles / min / μg |
Molecular Weight: |
This protein is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 25.8 kDa. The predicted N-terminus is Gln 29. DTT-reduced Protein migrates as 38 kDa in SDS-PAGE due to glycosylation. |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized |
Handling: |
Centrifuge the vial prior to opening. |