BioVision Products
Human CellExp™ CD58 /LFA-3, human recombinant
- SKU:
- 7427-50
Description
Human CellExp™ CD58 /LFA-3, human recombinant is available at Gentaur for Next week delivery.
Biomolecule/Target: CD58 /LFA-3
Alternates names: CD58, LFA3, Ag3
Synonyms: CD58, LFA3, Ag3
Background Information: CD antigen CD58 is also known as Lymphocyte function-associated antigen 3 (LFA-3). CD58 is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. CD58 /LFA3 is ligand of the T-lymphocyte CD2 glycoprotein, which binds to CD2 (LFA-2) on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells. Polymorphisms in the CD58 gene are associated with increased risk for multiple sclerosis.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
NCBI Gene Symbol: CD58
Gene ID: 965
NCBI Accession: P19256
Additional Information
Size: |
50 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
HEK293 cells |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
<1 EU/μg by LAL method |
Activity (Specifications/test method): |
N/A |
Biological activity: |
Measured in a cell proliferation assay using human CD3+ T cells in the presence of anti-CD3. The ED50 for this effect is typically 1 - 5 µg/ml in the presence of anti-CD3 immobilized at 25 ng/ml. |
Results: |
Measured in a cell proliferation assay using human CD3+ T cells in the presence of anti-CD3. The ED50 for this effect is typically 1 - 5 µg/ml in the presence of anti-CD3 immobilized at 25 ng/ml. |
Molecular Weight: |
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 22.3 kDa. The predicted N-terminus is Phe 29. DTT-reduced Protein migrates as 30-45 kDa due to different glycosylation. |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized |
Handling: |
Centrifuge the vial prior to opening. |