Description
Human CellExp™ Acid Sphingomyelinase, Human Recombinant is available at Gentaur for Next week delivery.
Catalyzes the hydrolysis of sphingomyelin to ceramide and phosphorylcholine
Biomolecule/Target:
Alternates names: Sphingomyelin phosphodiesterase, aSMase, acid sphingomyelinase, SMPD1, ASM, EC:3.1.4.12
Synonyms: Sphingomyelin phosphodiesterase, aSMase, acid sphingomyelinase, SMPD1, ASM, EC:3.1.4.12
Background Information: Sphingomyelin phosphodiesterase, which is encoded by the SMPD1 gene, is also known as acid sphingomyelinase or aSMase. There are two types of sphingomyelinases: ASM (acid), and NSM (neutral). ASM / aSMase can catalyze the hydrolysis of sphingomyelin to ceramide and phosphorylcholine with cofactor Zn2+. Ceramide, a bioactive lipid, has emerged as an important signaling molecule involved in a variety of cellular processes such as cell differentiation, apoptosis, and proliferation. Mutations in the SMPD1 gene cause Niemann–Pick disease types A and B due to deficiency in hydrolyzing sphingomyelin to ceramide. Activation of ASM can be achieved by the removal of its C terminal cysteine residue or C-terminal truncation. BioVision’s recombinant human ASM was expressed from HEK293 cells without the last three C terminal residues, and is therefore constitutively active.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in distilled water. Do not vortex.
NCBI Gene Symbol: SMPD1
Gene ID: 6609
NCBI Accession: P17405
Additional Information
Size: |
50 µg |
Adwords: |
False |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
True |
Source: |
HEK 293 cells |
Purity by SDS-PAGE: |
> 95% |
Assay: |
SDS-PAGE. |
Activity (Specifications/test method): |
The specific activity is >2 U/mg. Measured by its ability to cleave 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine (HNPPC, freshly dissolved in DMSO) which can be detected at 420 nm. |
Biological activity: |
The specific activity is >2 U/mg. Measured by its ability to cleave 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine (HNPPC, freshly dissolved in DMSO) which can be detected at 420 nm. |
Molecular Weight: |
68 kDa (C-terminal 8×His tag) |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Appearance: |
Lyophilized powder |
Handling: |
Centrifuge the vial prior to opening. |