BioVision Products
Human CellExp™ LIF, human recombinant
- SKU:
- 7267-10
Description
Human CellExp™ LIF, human recombinant is available at Gentaur for Next week delivery.
Affects cell growth and development
Biomolecule/Target: LIF
Alternates names: LIF, CDF, DIA, HILDA, MLPLI
Synonyms: LIF, CDF, DIA, HILDA, MLPLI
Background Information: Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
NCBI Gene Symbol: LIF
Gene ID: 3976
NCBI Accession: P15018
Additional Information
Size: |
10 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
HEK 293 cells |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
< 0.1 ng/μg of protein (<1EU/μg) by LAL method |
Activity (Specifications/test method): |
Determined by its ability to stimulate the proliferation of human TF-1 cells. The ED50 <0.1 ng/ml, corresponding to a specific activity of >1X10^7 units/mg. |
Biological activity: |
Determined by its ability to stimulate the proliferation of human TF-1 cells. The ED50 <0.1 ng/ml, corresponding to a specific activity of >1X10^7 units/mg. |
Results: |
N/A |
Molecular Weight: |
Calculated MW of 19.9 kDa with Gly-Pro at the N- terminus. The predicted N-terminus is Ser 23. DTT-reduced Protein migrates as 35-45 kDa. |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized powder |
Handling: |
Centrifuge the vial prior to opening. |