Human CellExp™ LIF, human recombinant

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SKU:
7267-10
$183
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Description

Human CellExp™ LIF, human recombinant is available at Gentaur for Next week delivery.

Affects cell growth and development

Biomolecule/Target: LIF

Alternates names: LIF, CDF, DIA, HILDA, MLPLI

Synonyms: LIF, CDF, DIA, HILDA, MLPLI

Background Information: Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

NCBI Gene Symbol: LIF

Gene ID: 3976

NCBI Accession: P15018

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Additional Information

Size:
10 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Human
Recombinant:
Yes
Source:
HEK 293 cells
Purity by SDS-PAGE:
≥95%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
N/A
Endotoxin Level:
< 0.1 ng/μg of protein (<1EU/μg) by LAL method
Activity (Specifications/test method):
Determined by its ability to stimulate the proliferation of human TF-1 cells. The ED50 <0.1 ng/ml, corresponding to a specific activity of >1X10^7 units/mg.
Biological activity:
Determined by its ability to stimulate the proliferation of human TF-1 cells. The ED50 <0.1 ng/ml, corresponding to a specific activity of >1X10^7 units/mg.
Results:
N/A
Molecular Weight:
Calculated MW of 19.9 kDa with Gly-Pro at the N- terminus. The predicted N-terminus is Ser 23. DTT-reduced Protein migrates as 35-45 kDa.
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
N/A
Appearance:
Lyophilized powder
Handling:
Centrifuge the vial prior to opening.
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Human CellExp™ LIF, mouse recombinant

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$183
Human CellExp™ LIF, mouse recombinant is available at Gentaur for Next week delivery. Affects cell growth and development Biomolecule/Target: LIF Alternates names: LIF, CDF, DIA, HILDA, MLPLI ...
7268-10