Alkaline phosphatase, mouse recombinant

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SKU:
P1098-10
€185
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Description

Alkaline phosphatase, mouse recombinant is available at Gentaur for Next week delivery.

A hydrolase enzyme responsible for removing phosphate groups

Biomolecule/Target: Alpl

Alternates names: Alkaline phosphatase, tissue-nonspecific isozyme, Alpl, Akp-2, Akp2, ALP, APTNAP, TNAP, TNSALP, HOPS

Synonyms: Alkaline phosphatase, tissue-nonspecific isozyme, Alpl, Akp-2, Akp2, ALP, APTNAP, TNAP, TNSALP, HOPS

Background Information: Alpl, also known as alkaline phosphatase, tissue-nonspecific isozyme, is one of alkaline phosphatases. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue-nonspecific). The product of this gene is a membrane-bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. A proposed function of this form of the enzyme is matrix mineralization. Recombinant mouse Alpl, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Reconstitution Instructions: N/A

NCBI Gene Symbol: Aplp

Gene ID: 11647

NCBI Accession: P09242

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Additional Information

Size:
10 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Mouse
Recombinant:
Yes
Source:
Insect cell (Baculovirus)
Purity by SDS-PAGE:
≥95%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
N/A
Endotoxin Level:
< 1 EU/μg
Activity (Specifications/test method):
Measured by its ability to hydrolyze 1pmole of 4-Methylumbelliferyl phosphate to phosphate and 4-Methylumbelliferone per minute at pH 8.8 at 25C.
Biological activity:
Specific activity is > 46,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of 4-Methylumbelliferyl phosphate to phosphate and 4-Methylumbelliferone per minute at pH 8.8 at 25C.
Results:
> 46,000 pmol/min/ug
Molecular Weight:
54.5 kDa
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
0.5 mg/ml
Appearance:
Liquid
Handling:
Centrifuge the vial prior to opening.
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