BioVision Proteins
Alkaline phosphatase, mouse recombinant
- SKU:
- P1098-10
Description
Alkaline phosphatase, mouse recombinant is available at Gentaur for Next week delivery.
A hydrolase enzyme responsible for removing phosphate groups
Biomolecule/Target: Alpl
Alternates names: Alkaline phosphatase, tissue-nonspecific isozyme, Alpl, Akp-2, Akp2, ALP, APTNAP, TNAP, TNSALP, HOPS
Synonyms: Alkaline phosphatase, tissue-nonspecific isozyme, Alpl, Akp-2, Akp2, ALP, APTNAP, TNAP, TNSALP, HOPS
Background Information: Alpl, also known as alkaline phosphatase, tissue-nonspecific isozyme, is one of alkaline phosphatases. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue-nonspecific). The product of this gene is a membrane-bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. A proposed function of this form of the enzyme is matrix mineralization. Recombinant mouse Alpl, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Reconstitution Instructions: N/A
NCBI Gene Symbol: Aplp
Gene ID: 11647
NCBI Accession: P09242
Additional Information
Size: |
10 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Mouse |
Recombinant: |
Yes |
Source: |
Insect cell (Baculovirus) |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
< 1 EU/μg |
Activity (Specifications/test method): |
Measured by its ability to hydrolyze 1pmole of 4-Methylumbelliferyl phosphate to phosphate and 4-Methylumbelliferone per minute at pH 8.8 at 25C. |
Biological activity: |
Specific activity is > 46,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of 4-Methylumbelliferyl phosphate to phosphate and 4-Methylumbelliferone per minute at pH 8.8 at 25C. |
Results: |
> 46,000 pmol/min/ug |
Molecular Weight: |
54.5 kDa |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
0.5 mg/ml |
Appearance: |
Liquid |
Handling: |
Centrifuge the vial prior to opening. |