BioVision Proteins
Alkaline phosphatase, placental, human recombinant
- SKU:
- P1044-50
Description
Alkaline phosphatase, placental, human recombinant is available at Gentaur for Next week delivery.
A hydrolase enzyme responsible for removing phosphate groups
Biomolecule/Target: ALPP
Alternates names: ALPP, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1
Synonyms: ALPP, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1
Background Information: ALPP, also known as alkaline phosphatase, placental, is a family of dimeric metalloenzymes catalyzing the hydrolysis of monoesters of phosphoric acid. Also, this protein catalyzes a transphosphorylation reaction in the presence of large concentrations of phosphate acceptors. It occurs widely in nature, and are found in many organisms from Escherichia coli to man. Most alkaline phosphatases are homodimeric enzymes and each catalytic site contains three metal ions (two Zn and one Mg), that are necessary for enzymatic activity. Recombinant human ALPP, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Reconstitution Instructions: N/A
NCBI Gene Symbol: ALPP
Gene ID: 250
NCBI Accession: P05187
Additional Information
Size: |
50 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
Baculovirus |
Purity by SDS-PAGE: |
> 95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
< 1.0 EU per/μg |
Activity (Specifications/test method): |
Measured by its ablity to hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C. |
Biological activity: |
Specific activity is > 2,500 units/mg, and is defined as the amount of enzyme that hydrolyzes 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C. |
Results: |
> 2,500 units/mg |
Molecular Weight: |
53.9 kDa |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Liquid |
Handling: |
Centrifuge the vial prior to opening. |