Active SIRT2, human recombinant

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SKU:
7698-50
$183
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Description

Active SIRT2, human recombinant is available at Gentaur for Next week delivery.

Human SIRT2 from E.Coli, AOF, ≥90% pure, biologically active.

Biomolecule/Target: SIRT2

Alternates names: NAD-dependent protein deacetylase sirtuin-2, Regulatory protein SIR2 homolog 2, SIR2-like protein 2, SIR2L, SIR2L2.

Synonyms: NAD-dependent protein deacetylase sirtuin-2, Regulatory protein SIR2 homolog 2, SIR2-like protein 2, SIR2L, SIR2L2.

Background Information: Sirtuins function as intracellular regulatory proteins. Human Sirtuin 2 is a member of the class III histone deacetylases (HDACs) and has been implicated in many cellular processes that include histone deacetylation, gene silencing, chromosomal stability, and aging. Human SIRT2 is a cytoplasmic protein responsible for the deacetylation of histone H4 and a-tubulin, a modification important for controlling the cell cycle. Specifically, SIRT2 protein co-localizes with HDAC6 and microtubules and functions as a mitotic checkpoint in preventing chromosomal instability that can lead to hyperploid cells. SIRT2 is found in many tissues, but is specifically enriched in skeletal muscle, the heart, and in oligodendroglia cells in the brain. The enzymatic activity of class III HDACs is nicotinamide adenine dinucleotide (0.1mM NAD+) dependent and insensitive to HDAC inhibitor trichostatin A.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile dH2O to a concentration of 0.1 – 1 mg/ml and let the lyophilized pellet dissolve completely. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use. For long term storage, it is recommended to add a carrier protein (0.1% BSA). Avoid multiple freeze/thaw cycles.

NCBI Gene Symbol: SIRT2

Gene ID: 22933

NCBI Accession: Q8IXJ6

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Additional Information

Size:
50 µg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Human
Recombinant:
Yes
Source:
E. coli.
Purity by SDS-PAGE:
≥90%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
HPLC
Endotoxin Level:
N/A
Activity (Specifications/test method):
Activity of SIRT2 was tested with BioVision’s Sirtuin Activity assay kit, Cat # K324-100, using the substrate Arg-His-Lys-Lys(Ac)-AFC, which is a fluorogenic, acetylated peptide based on residues 379-382 of p53.
Biological activity:
≥ 4 mU/mg
Results:
≥ 4 mU/mg
Molecular Weight:
Human SIRT2 is a 60 kDa (2 to 389 aa + NT His Tag) protein
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
N/A
Appearance:
Sirtuin 2 (SIRT2) with a His-tag is supplied as lyophilized powder.
Handling:
Centrifuge the vial prior to opening.
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