BioVision Proteins
Active D-2-Hydroxyglutaratate Dehydrogenase (D2HGDH), Recombinant
- SKU:
- P1001-50
Description
Active D-2-Hydroxyglutaratate Dehydrogenase (D2HGDH), Recombinant is available at Gentaur for Next week delivery.
D2HGDH is an active protein that catalyzes the oxidation of D-2-hydroxyglutarate to alpha-ketoglutarate.
Biomolecule/Target: D2HGDH
Alternates names: D-2-hydroxyglutarate dehydrogenase, D2HGDH, D2HGD
Synonyms: D-2-hydroxyglutarate dehydrogenase, D2HGDH, D2HGD
Background Information: D-2-hydroxyglutarate (D2HG) level is significantly increased in metabolic diseases and various cancers such as acute myeloid leukemia. Studies suggest that the detection of D2HG serves as a biomarker assay related to IDH (isocitrate dehydrogenase) mutations. D2HGDH is a special NAD-dependent enzyme which reacts with D2HG specifically and converts D2HG to α-ketoglutarate. D2HGDH is a key enzyme to distinguish between two metabolites, D2HG and L-2-hydroxyglutarate (L2HG), during biomarker assays.
Reconstitution Instructions: Reconstitute in water to a concentration of 0.1-5 mg/ml. The solution can be diluted into other aqueous buffers and stored at –20°C for future use.
NCBI Gene Symbol: D2HGDH
Gene ID: N/A
NCBI Accession: N/A
Additional Information
Size: |
50 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Acidaminococcus fermentans |
Recombinant: |
Yes |
Source: |
E. coli |
Purity by SDS-PAGE: |
≥99% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
The specific activity of D2HGDH is ≥ 90,000 mU/mg. (Specific activity is tested by the D-2-Hydroxyglutarate Colorimetric Assay Kit (Cat. No. 213-100) (Unit Definition: One unit is the amount of enzyme that reduces 1.0 µmole of NAD+ to NADH per min. a |
Biological activity: |
N/A |
Results: |
N/A |
Molecular Weight: |
39 kDa |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Dry powder |
Handling: |
Centrifuge the vial prior to opening. |