BioVision Proteins
Recombinant E. coli, D-lactate Dehydrogenase
- SKU:
- P1053-50
Description
Recombinant E. coli, D-lactate Dehydrogenase is available at Gentaur for Next week delivery.
Biomolecule/Target: ldhA
Alternates names: ECK1377, hslF, hslI, htpH, JW1375
Synonyms: ECK1377, hslF, hslI, htpH, JW1375, IdhA
Background Information: Idha, also known as D-lactate dehydrogenase, belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. In enzymology, a D-lactate dehydrogenase (cytochrome) is an enzyme that catalyzes the chemical reaction. Thus, the two substrates of this enzyme are (D)-lactate and ferricytochrome c, whereas its two products are pyruvate and ferrocytochrome c. Recombinant E.coli IdhA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Reconstitution Instructions: N/A
NCBI Gene Symbol: ldhA
Gene ID: 946315
NCBI Accession: P52643
Additional Information
Size: |
50 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
E. coli |
Recombinant: |
Yes |
Source: |
E. coli |
Purity by SDS-PAGE: |
> 95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
Measured by its ability to convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C. |
Biological activity: |
Specific activity is > 200 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C. |
Results: |
> 200 units/mg |
Molecular Weight: |
39.1 kDa |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Liquid |
Handling: |
Centrifuge the vial prior to opening. |