Active Caspase-1, rat recombinant

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SKU:
7524-25
$183
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Description

Active Caspase-1, rat recombinant is available at Gentaur for Next week delivery.

A cysteine protease involved in apoptosis

Biomolecule/Target: Caspase-1

Alternates names: Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, IL-1BC, p45.

Synonyms: Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, IL-1BC, p45.

Background Information: Caspase-1 (also known as ICE) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kDa) and small (10 kDa) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of caspase-1 activation and the role of caspase-1 in apoptosis are poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive proenzyme form. The recombinant rat caspase-1 was expressed in E. coli as a single polypeptide of 284 amino acids with molecular weight of 34.6. The caspase-1 is purified and activated by proprietary techniques.

Reconstitution Instructions: Reconstitute to 1 unit per μl in PBS containing 15% glycerol.

NCBI Gene Symbol: Casp1

Gene ID: N/A

NCBI Accession: P43527

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Additional Information

Size:
25 units
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Rat
Recombinant:
Yes
Source:
E. coli
Purity by SDS-PAGE:
N/A
Assay:
N/A
Purity:
N/A
Assay 2:
N/A
Endotoxin Level:
N/A
Activity (Specifications/test method):
N/A
Biological activity:
The caspase-1 is routinely tested at BioVision for its ability to cleave these two substrates Ac-YVAD-pNA (Cat. #1104-200) or Ac-YVAD-AFC (1103-200).
Results:
>3000 units/mg
Molecular Weight:
~ 34.6 KDa
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
N/A
Appearance:
Semi-Dry
Handling:
Centrifuge the vial prior to opening.
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