Description
Recombinant EBOV VP40 is available at Gentaur for Next Week Delivery.
Product Type: protein
Host: N/A
Clonality: N/A
Applications: ELISA, WB
Description: Recombinant, tag-free, purified Ebola virus (EBOV) matrix protein (VP40) is
expressed in E. coli and the recombinant protein purified using FPLC.
Storage: -80°C. It is recommended to dispense single-use aliquots and store aliquots at -80°C to
avoid multiple freeze/thaw cycles.
Size: 100 µg of protein is supplied in HEPES buffer pH 7.5 containing sodium chloride, 5% glycerol
and 0.1% Triton-X, at a concentration of 1.385 mg/mL. The theoretical molecular weight of the
protein is ~35 kDa.
Relevance: Recombinant EBOV VP40 matrix protein provides a means as a control protein for
immunoassays and a tool to enhance Filovirus research.
SDS-PAGE & Western Blot: Quality control testing shows a prominent protein band below 40 kDa (VP40).
SDS-PAGE & Western Blot Detection:
(A) SDS-PAGE and stain demonstrating 5 ug and 1
ug (lanes 1-2) of EBOV VP40 protein under
denaturing and reducing conditions. MW denotes
Novex® Sharp pre-stained protein markers. (B)
Western blot detection of EBOV VP40 at 50 ng and
100 ng (lanes 1-2). EBOV VP40 was detected using
IBT’s polyclonal antibody at 50 ng/mL (Cat. #
0301-010) and anti-rabbit IgG-HRP conjugate,
followed by TMB membrane substrate.
ELISA Data:
Plate was coated with EBOV VP40 starting at 800
ng/well, serially diluted in DPBS. Washed plate
was detected using one dilution of a positive
control serum, followed with anti-IgG HRP
conjugate and TMB substrate. OD650 is reported.