Description
Mouse anti-EBOV VP40 mAb (5B12) is available at Gentaur for Next Week Delivery.
Product Type: monoclonal antibody
Host: Mouse
Clonality: monoclonal
Applications: ELISA, WB
Immunogen: purified recombinant Ebola virus VP40
(EBOV rVP40) produced in E. coli.
Description: Protein G purified mouse monoclonal antibody reactive to EBOV VP40. The antibody detects VP40 in virus-like particles (VLP) and recombinant
protein.
Supplied: 100 µg is supplied in buffer containing Histidine, sodium chloride and sucrose at a concentration of 0.872 mg/mL. No preservative is added.
Purification: Antibody is purified using immobilized protein G.
Clonality: Monoclonal of the IgG2b isotype
Relevance: The antibody can be used for detection of
EBOV VP40.
Recommended Dilutions:
ELISA: Assay-dependent dilution
Western Blot: Assay-dependent dilution. Internal QC
demonstrates good detection when using 5B12 mAb at a
use dilution of 10 ng/mL.
Storage: 2-3 weeks +4°C, -20°C long term Cross Reactivity: No cross reactivity was observed against Sudan Virus (SUDV) or Marburg Virus (MARV) rVP40 or VLP.
Western Blot Data:
Western blots were detected under reduced conditions
with anti-EBOV VP40 mAb 5B12 at 10 ng/mL and
visualized using an anti-mouse IgG-HRP conjugate and
TMB membrane substrate. rVP40 was loaded at 100 ng
per lane. EBOV rVP40 is visualized in lane 2. VLP was
loaded at 1 µg per lane. VP40 is detected in EBOV VLP in
lane 5.
ELISA Data:
rVP40 proteins and VLPs were diluted to 1 and 10 µg/mL,
respectively in PBS for plate coating. Anti-EBOV VP40
antibody 5B12 was serially diluted semi-log dilutions
from 10 µg/mL and incubated on the coated plates.
Washed plates were detected with anti-mouse IgG-HRP
conjugate and TMB substrate. OD650 is reported above.