Recombinant EBOV Soluble GP (sGP)

Recombinant EBOV Soluble GP (sGP) is available at Gentaur for Next Week Delivery.

Product Type: protein

Host: N/A

Clonality: N/A

Applications: ELISA, WB

Description: Mature, recombinant Ebola Virus Soluble Glycoprotein (EBOV sGP) containing a C-terminal HIS
tag, is supplied as purified protein. EBOV sGP is produced in mammalian cells and is purified by FPLC.

Storage: 2-3 weeks at -20◦C, -80◦C long term.

Size: 100 µg of protein is supplied in PBS (supplemented with glycerol, arginine and glutamic acid) at a
concentration of 1.864 mg/mL. The theoretical molecular weight of the protein is ~43 kDa. Because of
the glycosylated nature of this protein, migration in an SDS-PAGE gel is slowed resulting in a broad, diffuse band representing differing glycosylation forms.

Relevance: Recombinant glycoprotein provides a means for antibody development, control protein for testing,
and a tool to enhance research.

Western Blot: Quality control testing demonstrates strong detection of EBOV soluble GP under reduced and
non-reduced conditions down to 50 ng when using IBT’s rabbit anti-EBOV sGP polyclonal antibody (cat 0365-
001) at 0.1 µg/mL.

SDS-PAGE & Western Blot Detection

(Panels A and B) SDS-PAGE demonstrating 5 ug and 1 ug
(lane 1 and 2, respectively) of EBOV sGP protein (A)
under reducing and denaturing conditions and (B) under
non-reducing and denaturing conditions.
(Panel C) Western blot detection of EBOV sGP reduced
and denatured at 200 ng, 100 ng, and 50 ng (lanes 1-3,
respectively). EBOV sGP was detected using IBT’s rabbit
anti-EBOV sGP polyclonal antibody at 0.1 ug/mL and
goat anti-rabbit IgG-HRP conjugate, followed by TMB
substrate.

ELISA Data

Plate was coated with EBOV sGP starting at 400 ng/well,
serially diluted in DPBS. Washed plate was detected
using one dilution of rabbit anti-EBOV sGP antibody
followed with goat anti-rabbit IgG-HRP conjugate and
TMB substrate. The OD650 reported shows the response
of the rabbit anti-EBOV sGP antibody against the titrated
EBOV sGP