EBOV Soluble GP (sGP) ELISA kit

EBOV Soluble GP (sGP) ELISA kit is available at Gentaur for Next Week Delivery.

Product Type: kit

Host: N/A

Clonality: N/A

Applications: ELISA

1. Purpose:
For the quantitative measurement of EBOV soluble glycoprotein in
mouse and non-human primate sera
2. Reagents supplied:

3. Reagents required but not included in the kit:

• DPBS 1X, sterile (MediaTech/Corning cat# 21-031-CM) stored at
  ambient temperature, for diluting coating antigen.
• StartingBlock T20 (PBS) Blocking Buffer (Pierce/Thermo cat# 37539)
  stored at 2-8C, for blocking and as diluent for standard, samples,
  detection antibody, and tertiary antibody
• DPBS powder (MediaTech/Corning cat# 55-031-PB) stored at 2-8C,
  for preparing ELISA Wash Buffer
• TWEEN-20 (Acros cat# 23336-0010), stored at ambient
  temperature, for preparing ELISA Wash Buffer Deionized water

4. Materials required but not included in the kit:

• MaxiSorp flat bottom, polystyrene, 96-well plates (Nunc cat#
  439454)
• Polypropylene TiterTubes, maximum volume for each tube = 1 mL
  (Bio-Rad cat# 223-9391) or equivalent, used to prepare standard
  and sample dilutions
• Microplate sealing film
• Polypropylene 15 mL and 50 mL conical tubes
• Reagent reservoirs
• Absorbent papers

5. Equipment required but not included in the kit:

•  Automatic plate washer (example: BioTek Elx450)
•  Plate reader with capability of measuring absorbance at 650 nm
  (example: Molecular Devices plate reader)
•  Software for graphing the standard as a 4PL curve and for calculating
  the unknown samples from the standard curve (example: Softmax
  software)
•  Single-channel and multi-channel pipettes

6. Assay Procedure:

1.  Prepare Capture antibody solution
   • Briefly spin the Capture Antibody vial and gently mix
   by pipetting up and down
   • Dilute 1:96 in DPBS 1X to target 4 µg/mL
   • Example: For one full plate, add 116 µL Capture
   antibody to 11 mL of DPBS 1X 
2. Add 100 µL/well of Capture antibody solution to the MaxiSorp
   plate. Cover plate using plate sealing film. Incubate covered
   plate overnight at 2-8C.
3.  The following day, equilibrate plate and StartingBlock Buffer to
   ambient temperature for at least 15 min.
4.  Empty contents from the plate and wash 3 times (each time 300
   µL/well) with Wash Buffer using an automatic plate washer or
   multi-channel pipette.
5.  Add 200 µL/well of StartingBlock Buffer to block non-specific
   binding. Incubate for at least 45 min at ambient temperature.
6.  During blocking step, prepare dilutions of STANDARD and
   UNKNOWN test samples in TiterTubes.