Mouse anti-EBOV VP40 mAb (3G5)

Mouse anti-EBOV VP40 mAb (3G5) is available at Gentaur for Next Week Delivery.

Product Type: monoclonal antibody

Host: Mouse

Applications: ELISA, WB

Immunogen: purified recombinant Ebola virus VP40 (EBOV rVP40) produced in E. coli.

Description: Protein G purified mouse monoclonal antibody reactive to EBOV VP40. The antibody detects VP40 in virus-like particles (VLP) and recombinant
protein.

Supplied: 100 µg is supplied in buffer containing Histidine, sodium chloride and sucrose at a concentration of 0.718 mg/mL. No preservative is
added.

Purification: Antibody is purified using immobilized protein G.

Clonality: Monoclonal of the IgG1 isotype

Relevance: The antibody can be used for detection of EBOV VP40.

Recommended Dilutions:
ELISA: Assay-dependent dilution
Western Blot: Assay-dependent dilution. Internal QC demonstrates good detection when using 3G5 mAb at a use dilution of 10 ng/mL.

Storage: 2-3 weeks +4°C, -20°C long term Cross Reactivity: Cross reactivity was observed against Sudan Virus (SUDV) VP40 in VLP and SUDV rVP40. No cross reactivity was observed with Marburg Virus
(MARV) VP40 in VLP or MARV rVP40.

Western Blot Data:

Western blots were detected under reduced conditions
with anti-EBOV VP40 mAb 3G5 at 10 ng/mL and
visualized using an anti-mouse IgG-HRP conjugate and
TMB membrane substrate. rVP40 was loaded at 100 ng
per lane. SUDV rVP40 and EBOV rVP40 are visualized in
lanes 1 and 2, respectively. VLP was loaded at 1 µg per
lane. VP40 is detected in SUDV VLP (lane 4) and EBOV
VLP (lane 5).

ELISA Data:

rVP40 proteins and VLPs were diluted to 1 and 10 µg/mL,
respectively in PBS for plate coating. Anti-EBOV VP40
antibody 3G5 was serially diluted semi-log dilutions from
10 µg/mL and incubated on the coated plates. Washed
plates were detected with anti-mouse IgG-HRP conjugate
and TMB substrate. OD650 is reported above.