CD-45 Antibody (CT)

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SKU:
6734-100
€245
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Description

CD-45 Antibody (CT)is available at Gentaur for Next week Delivery.

Description: CD45 has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45 are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5 and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45, while the intracellular domain is conserved. CD45 functions as a phosphotyrosine phosphatase, a vital component for efficient tyrosine phosphorylation induction by the TCR/CD3 complex. The tyrosine phosphatase activity of CD45 is contained within the conserved intracellular domain. Src and Syk family protein tyrosine kinases are utilized by the TCR/CD3 complex to initiate signaling cascades. Several members of these two families, including Lck, Fyn and ZAP-70, have been implicated as physiological substrates of CD45.

Immunogen Sequence:This CD45 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1245-1275 amino acids from the C-terminal region of human CD45.

Target Alternative Name:PTPRC; CD45; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; T200; Flags: Precursor.

Species Reactivities:Human

Tag Line:Polyclonal Antibody to detect CD-45 in human samples

Formulation:100 µl of antibody in PBS with 0.09% (W/V) sodium azide

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Application:Western blot, IHC, FACS

Positive Control:Western blot: Jurkat cell lysate, IHC: human tonsil tissue, FACS: Jurkat cells

Application And Usages:Western blot: ~1:1000, IHC: ~1:10–1:50, FACS: ~1:10-1:50.

Handling:The antibody solution should be gently mixed before use.

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Additional Information

Size:
100 μl
Antibody Target:
CD-45
Category:
CD-45
Lot Number:
9D226734
Host:
Rabbit
Antibody Type:
Polyclonal
Isotype:
Rabbit IgG
Accession Number:
P08575
Gene Id:
5788
Concentration:
N/A
Appearance:
Colorless liquid
Form:
Liquid
Storage Condition:
-20 °C
Shipping Condition:
gel pack
Shelf Life:
12 months
Country of Animal Origin:
USA
Country of Manufacture:
USA
Usage:
For Research Use Only! Not to be used in humans.
Western Blot Verified:
Verified
Immunocytochemistry Verified:
Verified
Immunofluorescence Verified:
Not Verified
Immunoprecipitation Verified:
Not Verified
ELISA Verified:
Not Verified
ChIP Verified:
Not Verified
Flow Cytometry Verified:
Verified
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