BioVision Proteins
Single Stranded DNA Binding Protein, E. coli
- SKU:
- 9220-50
Description
Single Stranded DNA Binding Protein, E. coli is available at Gentaur for Next week delivery.
A homo-tetrameric protein, which binds onto single stranded DNA to coordinate replication, repair, and recombinatio
Biomolecule/Target:
Alternates names: SSB,Single- Stranded DNA Binding Protein, E. coli
Synonyms: SSB,Single- Stranded DNA Binding Protein, E. coli
Background Information: Single Stranded DNA Binding Protein (SSB) is a homo-tetrameric protein, which binds onto single stranded DNA (ssDNA) to coordinate replication, repair, and recombination. It possesses a N-terminal DNA binding core and a C-terminal protein-protein interacting tail. ssDNA can form secondary structures and SSB prevents their formations. SSB can be added to PCR and DNA sequencing reactions to enhance the DNA polymerases processivity by allowing them to access their substrate more easily.
Reconstitution Instructions: N/A
NCBI Gene Symbol:
Additional Information
Size: |
50 μg |
Adwords: |
FAUX |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Recombinant: |
VRAI |
Source: |
E. coli strain containing an overproducing clone of E. coli SSB protein. |
Purity by SDS-PAGE: |
≥ 99% by SEC and SDS-PAGE analysis |
Assay: |
The activity of SSB protein was determined using Gel Shift Assay. Varying amounts of SSB (150 ng to 86 μg) were able to bind onto 22 fmol of M13mp18 ssDNA in a 20 μl reaction volume and affect ssDNA mobility. Sample was loaded using 10x Orange DNA Lo |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
N/A |
Biological activity: |
N/A |
Results: |
N/A |
Molecular Weight: |
18.9 kDa |
Storage Temperature: |
-20°C |
Shelf Life: |
24 months |
Concentration: |
Lot specific |
Appearance: |
Liquid |
Handling: |
Centrifuge the vial prior to opening. |
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