Single Stranded DNA Binding Protein, E. coli

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SKU:
9220-50
€185
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Description

Single Stranded DNA Binding Protein, E. coli is available at Gentaur for Next week delivery.

A homo-tetrameric protein, which binds onto single stranded DNA to coordinate replication, repair, and recombinatio

Biomolecule/Target:

Alternates names: SSB,Single- Stranded DNA Binding Protein, E. coli

Synonyms: SSB,Single- Stranded DNA Binding Protein, E. coli

Background Information: Single Stranded DNA Binding Protein (SSB) is a homo-tetrameric protein, which binds onto single stranded DNA (ssDNA) to coordinate replication, repair, and recombination. It possesses a N-terminal DNA binding core and a C-terminal protein-protein interacting tail. ssDNA can form secondary structures and SSB prevents their formations. SSB can be added to PCR and DNA sequencing reactions to enhance the DNA polymerases processivity by allowing them to access their substrate more easily.

Reconstitution Instructions: N/A

NCBI Gene Symbol:

Gene ID:

NCBI Accession:

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Additional Information

Size:
50 μg
Adwords:
FAUX
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Recombinant:
VRAI
Source:
E. coli strain containing an overproducing clone of E. coli SSB protein.
Purity by SDS-PAGE:
≥ 99% by SEC and SDS-PAGE analysis
Assay:
The activity of SSB protein was determined using Gel Shift Assay. Varying amounts of SSB (150 ng to 86 μg) were able to bind onto 22 fmol of M13mp18 ssDNA in a 20 μl reaction volume and affect ssDNA mobility. Sample was loaded using 10x Orange DNA Lo
Purity:
N/A
Assay 2:
N/A
Endotoxin Level:
N/A
Activity (Specifications/test method):
N/A
Biological activity:
N/A
Results:
N/A
Molecular Weight:
18.9 kDa
Storage Temperature:
-20°C
Shelf Life:
24 months
Concentration:
Lot specific
Appearance:
Liquid
Handling:
Centrifuge the vial prior to opening.
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