Description
PKCa, Active is available at Gentaur for Next week delivery.
Phosphorylate a wide variety of protein targets
Biomolecule/Target:
Alternates names: PKC, Protein kinase C alpha (PKCα),
Synonyms: PKC, Protein kinase C alpha (PKCα),
Background Information: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. PKC-α has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Coussens defined a new family of PKC-related genes and termed α, β, and γ (1). Latos-Bielenska refined the assignment of PRKCA1 to 17q22-q23.2 (2). Braz identified PKC-α as a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes in study of knockout mice (3).
Reconstitution Instructions: N/A
NCBI Gene Symbol: PKCA
Gene ID: 5578
NCBI Accession: P17252
Additional Information
Size: |
5 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Recombinant: |
Yes |
Source: |
Baculovirus (Sf9 insect cells) |
Purity by SDS-PAGE: |
≥90% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
HPLC |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
69 nmol phosphate incorporated into CREBtide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay). |
Biological activity: |
N/A |
Results: |
N/A |
Molecular Weight: |
103.0 kDa |
Storage Temperature: |
-80°C |
Shelf Life: |
12 months |
Concentration: |
5µg/50 µl |
Appearance: |
Liquid |
Handling: |
Centrifuge the vial prior to opening. |