Description
PHPT1, human recombinant is available at Gentaur for Next week delivery.
Enzyme that exhibits phosphohistidine phosphatase activity
Biomolecule/Target: PHPT1
Alternates names: bA216L13.10, CGI-202, DKFZp564M173, HSPC141, PHP14, RP11-216L13.10, 14 kDa phosphohistidine phosphatase
Synonyms: bA216L13.10, CGI-202, DKFZp564M173, HSPC141, PHP14, RP11-216L13.10, 14 kDa phosphohistidine phosphatase
Background Information: PHPT1 is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides. Recombinant human PHPT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Reconstitution Instructions: N/A
NCBI Gene Symbol: PHPT1
Gene ID: 29085
NCBI Accession: Q9NRX4
Additional Information
Size: |
50 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
E.coli |
Purity by SDS-PAGE: |
> 95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
Measured by its ability to hydrolyze 1nmol of p-nitrophenyl phosphate per minute at pH7.5 at 37°C. |
Biological activity: |
Specific activity is >120 units/mg |
Results: |
>120 units/mg |
Molecular Weight: |
15.9 kDa |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Liquid |
Handling: |
Centrifuge the vial prior to opening. |
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