PHPT1, human recombinant

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SKU:
P1181-50
€185
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Description

PHPT1, human recombinant is available at Gentaur for Next week delivery.

Enzyme that exhibits phosphohistidine phosphatase activity

Biomolecule/Target: PHPT1

Alternates names: bA216L13.10, CGI-202, DKFZp564M173, HSPC141, PHP14, RP11-216L13.10, 14 kDa phosphohistidine phosphatase

Synonyms: bA216L13.10, CGI-202, DKFZp564M173, HSPC141, PHP14, RP11-216L13.10, 14 kDa phosphohistidine phosphatase

Background Information: PHPT1 is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides. Recombinant human PHPT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Reconstitution Instructions: N/A

NCBI Gene Symbol: PHPT1

Gene ID: 29085

NCBI Accession: Q9NRX4

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Additional Information

Size:
50 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Human
Recombinant:
Yes
Source:
E.coli
Purity by SDS-PAGE:
> 95%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
N/A
Endotoxin Level:
N/A
Activity (Specifications/test method):
Measured by its ability to hydrolyze 1nmol of p-nitrophenyl phosphate per minute at pH7.5 at 37°C.
Biological activity:
Specific activity is >120 units/mg
Results:
>120 units/mg
Molecular Weight:
15.9 kDa
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
N/A
Appearance:
Liquid
Handling:
Centrifuge the vial prior to opening.
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