Noggin, human recombinant

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SKU:
4675-50
£135.37
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Description

Noggin, human recombinant is available at Gentaur for Next week delivery.

An inhibitor of TGF-β signal transduction

Biomolecule/Target:

Alternates names: NOG

Synonyms: none

Background Information: Noggin belongs to a group of diffusible proteins which bind to ligands of the TGF-β family and regulate their activity by inhibiting their access to signaling receptors. Noggin was originally identified as a BMP-4 antagonist whose action is critical for proper formation of the head and other dorsal structures. Consequently, Noggin has been shown to modulate the activities of other BMPs including BMP-2,-7,-13, and -14. Targeted deletion of Noggin in mice results in prenatal death and recessive phenotype displaying a severely malformed skeletal system. Conversely, transgenic mice over-expressing Noggin in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis. Recombinant human Noggin is a 23.1 kDa non-disulfide-linked homodimer consisting of a total of 206 amino acid residues.

Reconstitution Instructions: Reconstitute in H₂O to a concentration of 0.1 to 1.0 mg/ml. Note: Due to solubility reasons the protein should be kept at low pH. This solution can then be diluted into other aqueous buffers

NCBI Gene Symbol: NOG

Gene ID: 9241

NCBI Accession: Q13253

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Additional Information

Size:
50 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Human
Recombinant:
Yes
Source:
E. coli
Purity by SDS-PAGE:
≥95%
Assay:
SDS-PAGE
Purity:
≥95%
Assay 2:
HPLC
Endotoxin Level:
<0.1 ng/μg
Activity (Specifications/test method):
N/A
Biological activity:
Determined by its ability to inhibit 5 ng/ml of BMP-4-induced alkaline phosphatase production by ATDC chondrogenic cells. The expected ED₅₀ for the effect is 0.05-0.08 µg/ml of Noggin
Results:
0.05-0.08 µg/ml
Molecular Weight:
23.1 kDa
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
N/A
Appearance:
Lyophilized protein
Handling:
Centrifuge the vial prior to opening.
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