LIF, human recombinant

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SKU:
4923-50
$719.07
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Description

LIF, human recombinant is available at Gentaur for Next week delivery.

A cytokine that affects cell growth by inhibiting differentiation

Biomolecule/Target:

Alternates names: Leukemia Inhibitory Factor, Differentiation-stimulating factor, D factor, Melanoma-derived LPL inhibitor, INN=Emfilermin

Synonyms: Leukemia Inhibitory Factor

Background Information: Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. Human LIF is as active on human cells as is it is on mouse cells, though mouse LIF is about 1000 fold less active on human cells, than human LIF. Recombinant human LIF produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids and having a molecular mass of 19.7 kDa.

Reconstitution Instructions: Reconstitute in H₂O to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

NCBI Gene Symbol: LIF

Gene ID: 3976

NCBI Accession: P15018

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Additional Information

Size:
50 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Human
Recombinant:
Yes
Source:
E. coli
Purity by SDS-PAGE:
≥95%
Assay:
SDS-PAGE
Purity:
≥95%
Assay 2:
HPLC
Endotoxin Level:
<0.1 ng/μg
Activity (Specifications/test method):
N/A
Biological activity:
The ED₅₀ determined by the M1 cell differentiation assay is < 0.01 ng/ml, corresponding to a specific activity of 100MIU/mg.
Results:
N/A
Molecular Weight:
19.7 kDa
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
N/A
Appearance:
Lyophilized protein
Handling:
Centrifuge the vial prior to opening.
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