INS, 25-110 aa, His-tag, Human Recombinant

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SKU:
P1302-5
$183
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Description

INS, 25-110 aa, His-tag, Human Recombinant is available at Gentaur for Next week delivery.

Essential hormone for maintaining metabolic homeostasis

Biomolecule/Target: INS

Alternates names: Insulin preproprotein , IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10

Synonyms: Insulin preproprotein , IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10

Background Information: INS, also known as insulin preproprotein, is a biologically inactive precursor to the biologically active endocrine hormone insulin. Insulin is an essential hormone for maintaining metabolic homeostasis in the body. To make fully bioactive insulin, pancreatic beta cells initiate synthesis of INS. It is converted into proinsulin by signal peptidases, which remove its signal peptide from its N-terminus. Finally, proinsulin is converted into the bioactive hormone insulin by removal of the C-peptide. Recombinant human INS, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.

Reconstitution Instructions:

NCBI Gene Symbol: INS

Gene ID: 3630

NCBI Accession: NP_000198

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Additional Information

Size:
5 µg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Human
Recombinant:
Yes
Source:
E.coli
Purity by SDS-PAGE:
≥ 95%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
N/A
Endotoxin Level:
< 1.0 EU per 1ug of protein (determined by LAL method)
Biological activity:
Measured in a cell proliferation assay using MCF7 human breast cancer cells. The ED50 for this effect is less or equal to 4 ug/ml.
Results:
N/A
Molecular Weight:
11.8 kDa (109 aa), confirmed by MALDI-TOF
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
0.5 mg/ml (determined by Absorbance at 280nm)
Appearance:
Liquid
Handling:
Centrifuge the vial prior to opening.
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