BioVision Proteins
INS, 25-110 aa, His-tag, Human Recombinant
- SKU:
- P1302-5
Description
INS, 25-110 aa, His-tag, Human Recombinant is available at Gentaur for Next week delivery.
Essential hormone for maintaining metabolic homeostasis
Biomolecule/Target: INS
Alternates names: Insulin preproprotein , IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10
Synonyms: Insulin preproprotein , IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10
Background Information: INS, also known as insulin preproprotein, is a biologically inactive precursor to the biologically active endocrine hormone insulin. Insulin is an essential hormone for maintaining metabolic homeostasis in the body. To make fully bioactive insulin, pancreatic beta cells initiate synthesis of INS. It is converted into proinsulin by signal peptidases, which remove its signal peptide from its N-terminus. Finally, proinsulin is converted into the bioactive hormone insulin by removal of the C-peptide. Recombinant human INS, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.
Reconstitution Instructions:
NCBI Gene Symbol: INS
Gene ID: 3630
NCBI Accession: NP_000198
Additional Information
Size: |
5 µg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
E.coli |
Purity by SDS-PAGE: |
≥ 95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
< 1.0 EU per 1ug of protein (determined by LAL method) |
Biological activity: |
Measured in a cell proliferation assay using MCF7 human breast cancer cells. The ED50 for this effect is less or equal to 4 ug/ml. |
Results: |
N/A |
Molecular Weight: |
11.8 kDa (109 aa), confirmed by MALDI-TOF |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
0.5 mg/ml (determined by Absorbance at 280nm) |
Appearance: |
Liquid |
Handling: |
Centrifuge the vial prior to opening. |