Human Recombinant DHFR

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SKU:
6382-100
£135.37
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Description

Human Recombinant DHFR is available at Gentaur for Next week delivery.

Reduces dihydrofolic acid to tetrahydrofolic acid

Biomolecule/Target:

Alternates names: Dihydrofolate reductase.

Synonyms: Human Recombinant DHFR

Background Information: DHFR, also known as Dihydrofolate reductase, is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as electron donor, which can be converted to tetrahydrofolate cofactors used in 1-carbon transfer chemistry. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Human dihydrofolate reductase has been used in a study to investigate the stable expression of green fluorescent protein and the targeted disruption of thioredoxin peroxidase-1 gene in Babesia bovis. Human dihydrofolate reductase has also been used in a study to investigate the structural analysis of human dihydrofolate reductase as a binary complex.

Reconstitution Instructions: N/A

NCBI Gene Symbol: DHFR

Gene ID: 1719

NCBI Accession: P00374

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Additional Information

Size:
100 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Human
Recombinant:
Yes
Source:
E. Coli
Purity by SDS-PAGE:
≥95%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
N/A
Endotoxin Level:
< 1.0 EU per 1 μg of protein (determined by LAL method)
Activity (Specifications/test method):
N/A
Biological activity:
Specific activity is > 2000 pmol/min/ug, and is defined as the amount of enzyme that converts 1.0 pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25C.
Results:
1.5 - 2.5 units/ml
Molecular Weight:
23.6 kDa (207 aa, 1-187 aa + NT His-Tag)
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
1 mg/ml
Appearance:
Liquid
Handling:
Centrifuge the vial prior to opening.
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