Description
Human Recombinant DHFR is available at Gentaur for Next week delivery.
Reduces dihydrofolic acid to tetrahydrofolic acid
Biomolecule/Target:
Alternates names: Dihydrofolate reductase.
Synonyms: Human Recombinant DHFR
Background Information: DHFR, also known as Dihydrofolate reductase, is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as electron donor, which can be converted to tetrahydrofolate cofactors used in 1-carbon transfer chemistry. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Human dihydrofolate reductase has been used in a study to investigate the stable expression of green fluorescent protein and the targeted disruption of thioredoxin peroxidase-1 gene in Babesia bovis. Human dihydrofolate reductase has also been used in a study to investigate the structural analysis of human dihydrofolate reductase as a binary complex.
Reconstitution Instructions: N/A
NCBI Gene Symbol: DHFR
Gene ID: 1719
NCBI Accession: P00374
Additional Information
Size: |
100 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
E. Coli |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
< 1.0 EU per 1 μg of protein (determined by LAL method) |
Activity (Specifications/test method): |
N/A |
Biological activity: |
Specific activity is > 2000 pmol/min/ug, and is defined as the amount of enzyme that converts 1.0 pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25C. |
Results: |
1.5 - 2.5 units/ml |
Molecular Weight: |
23.6 kDa (207 aa, 1-187 aa + NT His-Tag) |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
1 mg/ml |
Appearance: |
Liquid |
Handling: |
Centrifuge the vial prior to opening. |