BioVision Products
Human CellExp™ PLGF/PIGF2/PGF, human recombinant
- SKU:
- 7238-10
Description
Human CellExp™ PLGF/PIGF2/PGF, human recombinant is available at Gentaur for Next week delivery.
A key molecule in angiogenesis and vasculogenesis
Biomolecule/Target: PLGF
Alternates names: PGF, PLGF, PlGF2, PlGF, PGFL, SHGC-10760
Synonyms: PGF, PLGF, PlGF2, PlGF, PGFL, SHGC-10760
Background Information: Placental growth factor (PGF) also known as vascular endothelial growth factor-related protein, PLGF and PlGF2, is a member of the VEGF (vascular endothelial growth factor) sub-family - a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. The main source of PGF during pregnancy is the placental trophoblast. PGF is also expressed in many other tissues, including the villous trophoblast. PGF is actived in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. PlGF2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
NCBI Gene Symbol: PLGF
Gene ID: 5228
NCBI Accession: P49763
Additional Information
Size: |
10 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
HEK 293 cells |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
HPLC |
Endotoxin Level: |
< 0.1 ng/μg of protein (<1EU/μg). |
Activity (Specifications/test method): |
Measured in a cell proliferation assay using MDA - MB – 231 human breast cancer cells. The ED50 for this effect is typically 1 - 10 µg/ml. |
Biological activity: |
Measured in a cell proliferation assay using MDA - MB – 231 human breast cancer cells. The ED50 for this effect is typically 1 - 10 µg/ml. |
Results: |
N/A |
Molecular Weight: |
The protein is fused with 6×His tag at the C-terminus, has a calculated MW of 20.2 kDa. The predicted N-terminus is Leu 19. DTT-reduced Protein migrates as 28-33 kDa due to glycosylation. |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized powder |
Handling: |
Centrifuge the vial prior to opening. |