BioVision Products
Human CellExp™ Osteoactivin / GPNMB, human recombinant
- SKU:
- 7513-10
Description
Human CellExp™ Osteoactivin / GPNMB, human recombinant is available at Gentaur for Next week delivery.
≥95% pure, active, human cell expressed, human recombinant Osteoactivin / GPNMB - acts as a downstream mediator of BMP-2.
Biomolecule/Target: Osteoactivin / GPNMB
Alternates names: GPNMB, HGFIN, NMB, Osteoactivin
Synonyms: GPNMB, HGFIN, NMB, Osteoactivin
Background Information: Transmembrane glycoprotein NMB (GPNMB) is also known as Transmembrane glycoprotein HGFIN, DC-HIL and Osteoactivin (OA), which belongs to the PMEL/NMB family. GPNMB contains one PKD domain. GPNMB is a transmembrane glycoprotein that is up-regulated in various cancer cells, including in glioblastoma multiforme and is expressed in many melanoma cells, as well as in tissue macrophages. GPNMB protein acts as a downstream mediator of BMP-2 effects on osteoblast differentiation and function. GPNMB participates in bone mineralization, and functions as a negative regulator of inflammation in macrophages.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
NCBI Gene Symbol: GPNMB
Gene ID: 10457
NCBI Accession: Q96F58
Additional Information
Size: |
10 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
HEK293 cells |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
<1 EU/μg by LAL method |
Activity (Specifications/test method): |
N/A |
Biological activity: |
Measured by the ability of the immobilized protein to induce pro-MMP-9 secretion by NHLF human normal lung fibroblasts. The ED50 for this effect is typically 4 - 16 µg/ml. |
Results: |
ED50 for this effect is typically 4 - 16 µg/ml |
Molecular Weight: |
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 52.9 kDa. The predicted N-terminus is Ala 22. DTT-reduced Protein migrates as 85-100 kDa due to glycosylation. |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized |
Handling: |
Centrifuge the vial prior to opening. |