BioVision Products
Human CellExp™ Lipocalin-2 / LCN2, human recombinant
- SKU:
- 7460-50
Description
Human CellExp™ Lipocalin-2 / LCN2, human recombinant is available at Gentaur for Next week delivery.
Functions as a growth factor and is upregulated during inflammation
Biomolecule/Target: Lipocalin-2
Alternates names: NGAL, LCN2, Lipocalin-2, 24p3, MSFI, Siderocalin; Oncogene 24p3; Neutrophil Gelatinase-associated Lipocalin; NGAL.
Synonyms: NGAL, LCN2, Lipocalin-2, 24p3, MSFI, Siderocalin; Oncogene 24p3; Neutrophil Gelatinase-associated Lipocalin; NGAL.
Background Information: Lipocalin-2 (LCN2), also known as oncogene 24p3 or neutrophil gelatinase-associated lipocalin (NGAL), MSFI, The binding of lipocalin-2 to bacterial siderophores is important in the innate immune response to bacterial infection. Upon encountering invading bacteria the toll-like receptors on immune cells stimulate the synthesis and secretion of lipocalin-2. Secreted lipocalin-2 then limits bacterial growth by sequestering iron-containing siderophores. Lipocalin-2 also functions as a growth factor. LCN2 is strongly upregulated during inflammation and is upregulated by interleukin 1 (but not TNF alpha) in humans. There are indications that some forms of acne could be caused due to the gene not being transcribed, and that Isotretinoin corrects this. NFAT3 (NFATc4) NFAT by blocking the expression of LCN2 inhibits breast carcinoma cell motility. Recent studies have revealed that NGAL plays an important role in the physiopathology of chronic myeloid leukaemia (CML) mediated by BCR-ABL.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
NCBI Gene Symbol: LCN2
Gene ID: 3934
NCBI Accession: P80188
Additional Information
Size: |
50 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
HEK293 cells |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
<1 EU/μg by LAL method |
Activity (Specifications/test method): |
Measured by its ability to bind Iron (III) dihydroxybenzoic acid. [Fe(DHBA)3]. The binding of Fe (DHBA)3 results in the quenching of Trp fluorescence in rhLCN2. rhLCN2 can bind >1.5 μM of Fe(DHBA)3. |
Biological activity: |
Measured by its ability to bind Iron (III) dihydroxybenzoic acid. [Fe(DHBA)3]. The binding of Fe (DHBA)3 results in the quenching of Trp fluorescence in rhLCN2. rhLCN2 can bind >1.5 μM of Fe(DHBA)3. |
Results: |
Measured by its ability to bind Iron (III) dihydroxybenzoic acid. [Fe(DHBA)3]. The binding of Fe (DHBA)3 results in the quenching of Trp fluorescence in rhLCN2. rhLCN2 can bind >1.5 μM of Fe(DHBA)3. |
Molecular Weight: |
This protein is fused with 6×his tag at C-terminal and has a calculated MW of 22 kDa. The predicted N-terminal is Gln 21. DTT-reduced protein migrates as 23-25 kDa. |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized |
Handling: |
Centrifuge the vial prior to opening. |